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BEGIN:VEVENT
UID:20260818T191149Z - 41417@eupv304
DTSTART;TZID=Europe/Brussels:20250911T160000
DTEND;TZID=Europe/Brussels:20250911T170000
CREATED:20260818T191149Z
DESCRIPTION:<a href="https://www.analis.com/event/beckman-webinar-extracell
 ular-vesicles-characterization-and-isolation-through-nanoscale-flow-cytome
 try-205/register">BECKMAN Webinar Extracellular vesicles characterization 
 and isolation through nanoscale flow cytometry</a>\nINFO & REGISTRATION Da
 te: September 11\, 2025 Time: 7:00 AM (PST)\, 10:00 AM (EST)\, 4:00 PM (CE
 T) Abstract Introduction: Extracellular Vesicles (EVs) are key mediators o
 f intercellular communication\, enclosed by a phospholipid bilayer and lac
 k the ability to replicate. Characterization of EVs is essential to elucid
 ate their cellular origins\, molecular cargo\, and functional roles in hea
 lth and disease. However\, the nanoscale size and low refractive index (RI
 ) present significant challenges for detection and size determination with
  conventional flow cytometers. Furthermore\, the use of standard polystyre
 ne beads for calibration complicates the analysis due to their significant
 ly higher light-scattering properties compared to EVs. The CytoFLEX nano r
 epresents a new platform for EV evaluation. The instrument is equipped wit
 h a multiple-scatter laser which increases the sensitivity for the detecti
 on of small EVs. By combining scatter-based detection with fluorescence an
 tibodies labelling\, size\, concentration\, and expression of EVs surface 
 markers can be measured simultaneously\, providing a more accurate and sen
 sitive approach to EVs analysis. Aim: The aim of this study was to calibra
 te the CytoFLEX nano flow cytometer for the detection and analysis of smal
 l EVs with diameters less than 300 nm. For this purpose\, the Cellarcus Ve
 sicle Analysis Kit (vFC™) and AcoDyes™ were employed. Following calibr
 ation\, plasma-derived EVs were analysed. Additionally\, nanoViS polystyre
 ne beads were used in combination with FCMPASS software to estimate the si
 ze distribution of EVs. Materials and Methods: The CytoFLEX nano is equipp
 ed with multiple scatter and fluorescence detection channels. Calibration 
 and standardization were performed using the vFC™ Kit\, which includes l
 ipid-based vesicle standards that closely mimic the refractive index (RI) 
 of native EVs\, along with two fluorescently [...]
DTSTAMP:20260818T191149Z
LOCATION:Teams Webinar\, Belgium
SUMMARY:BECKMAN Webinar Extracellular vesicles characterization and isolati
 on through nanoscale flow cytometry
X-ALT-DESC;FMTTYPE=text/html:<a href="https://www.analis.com/event/beckman-
 webinar-extracellular-vesicles-characterization-and-isolation-through-nano
 scale-flow-cytometry-205/register">BECKMAN Webinar Extracellular vesicles 
 characterization and isolation through nanoscale flow cytometry</a>\nINFO 
 & REGISTRATION Date: September 11\, 2025 Time: 7:00 AM (PST)\, 10:00 AM (E
 ST)\, 4:00 PM (CET) Abstract Introduction: Extracellular Vesicles (EVs) ar
 e key mediators of intercellular communication\, enclosed by a phospholipi
 d bilayer and lack the ability to replicate. Characterization of EVs is es
 sential to elucidate their cellular origins\, molecular cargo\, and functi
 onal roles in health and disease. However\, the nanoscale size and low ref
 ractive index (RI) present significant challenges for detection and size d
 etermination with conventional flow cytometers. Furthermore\, the use of s
 tandard polystyrene beads for calibration complicates the analysis due to 
 their significantly higher light-scattering properties compared to EVs. Th
 e CytoFLEX nano represents a new platform for EV evaluation. The instrumen
 t is equipped with a multiple-scatter laser which increases the sensitivit
 y for the detection of small EVs. By combining scatter-based detection wit
 h fluorescence antibodies labelling\, size\, concentration\, and expressio
 n of EVs surface markers can be measured simultaneously\, providing a more
  accurate and sensitive approach to EVs analysis. Aim: The aim of this stu
 dy was to calibrate the CytoFLEX nano flow cytometer for the detection and
  analysis of small EVs with diameters less than 300 nm. For this purpose\,
  the Cellarcus Vesicle Analysis Kit (vFC™) and AcoDyes™ were employed.
  Following calibration\, plasma-derived EVs were analysed. Additionally\, 
 nanoViS polystyrene beads were used in combination with FCMPASS software t
 o estimate the size distribution of EVs. Materials and Methods: The CytoFL
 EX nano is equipped with multiple scatter and fluorescence detection chann
 els. Calibration and standardization were performed using the vFC™ Kit\,
  which includes lipid-based vesicle standards that closely mimic the refra
 ctive index (RI) of native EVs\, along with two fluorescently [...]
END:VEVENT
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