Capillary Electrophoresis

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Découvrez la puissance et la vitesse de la technologie de séparation SCIEX
Les instruments d'électrophorèse capillaire SCIEX ont été développés pour répondre à vos défis analytiques d'aujourd'hui, tout en gardant un œil fermement tourné vers les exigences de demain.

Que votre laboratoire effectue la sélection de clones, l'analyse automatisée et quantitative de produits biologiques, la recherche protéomique, métabolomique ou génomique, ou les tests alimentaires et environnementaux, SCIEX est sûr d'avoir une solution de pointe pour vous.

Apprenez-en plus sur nos instruments d'électrophorèse capillaire, et voyez pourquoi les scientifiques du monde entier comptent sur la technologie SCIEX pour faire avancer leurs recherches.

Contactez-nous pour discuter de l'instrument le mieux adapté à vos besoins et à votre budget.

Système Sciex BioPhase 8800

BioPhase 8800 system

Système innovant qui facilite le traitement parallèle de 8 échantillons simultanément, tout en fournissant des analyses CE-SDS et CIEF sensibles. Il permet d'obtenir une précision sans compromis et des délais de réponse plus courts tout au long du processus de développement des produits biologiques.

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sciex PA 800 Plus Système d'analyse pharmaceutique

PA 800 Plus Pharmaceutical Analysis System

Plate-forme flexible pour de multiples essais analytiques avec un capillaire à température contrôlée pour une reproductibilité et une confiance maximales des données.

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sciex CESI 8000 Plus ESI-MS-Système à haute performance

CESI 8000 Plus ESI-MS-High-Performance System

Intègre l'électrophorèse capillaire et l'ionisation par électrospray (ESI) en un seul processus dynamique. Augmente considérablement l'efficacité, la vitesse et la sensibilité des séparations.

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sciex P/ACE™ MDQ Plus

P/ACE™ MDQ Plus

Systèmes d'électrophorèse capillaire automatisés et programmables conçus pour effectuer des séparations rapides à partir d'échantillons complexes.

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sciex GenomeLab GeXP™ Système d'analyse génétique

GenomeLab GeXP™ Genetic Analysis System

Tous vos besoins en matière d'analyses génétiques sur un seul instrument. Le GenomeLab GeXP prend en charge une variété d'applications, y compris le séquençage de l'ADN, l'analyse de fragments et l'expression génique multiplex.

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Nos produits

SCIEX

Advanced cIEF Starter Kit

Reference : ABS-A80976

Accurate determination of a protein’s charge heterogeneity helps establish identity and stability. Capillary Isoelectric Focusing (cIEF) is a powerful technique that allows quantitative analysis of a protein’s isoelectric point (pI). In cIEF, a mixture of sample and ampholyte is introduced into a capillary and subjected to electrophoretic separation. In this process, a pH gradient through which analytes migrate to their respective pI is formed. 

The PA 800 Plus Pharmaceutical Analysis System automates the advanced cIEF technology necessary to successfully achieve high precision and quantitative separations. The use of optimized universal methods and synthetic pI markers attains the highest levels of precision in pI calculation and direct isoform quantitation with on-line UV detection.

This kit provides reagent volumes for analysis of 100 samples and includes: 
• eCap Neutral Capillary, 1 unit 
• cIEF gel 
• cIEF Peptide Marker Kit, 5 vials (pI 4.1, pI 5.5, pI 7.0, pI 9.5, pI 10.0), 240 µL each 
• Sample Loading Solution (SLS), 6 mL 
• CE Grade Water 
 
 
NOTE: Additional reagents from other vendors are required for this application. Methods and necessary supplies can be referenced in the following AIBs, available for download at www.sciex.com:
• A-11634: Identification of System Parameters Critical for High-Performance cIEF
• A-12015: A Robust cIEF Method: Intermediate Precision for the pH 5-7 Range
• A-12026: High-Resolution cIEF of Therapeutic Monoclonal Antibodies: A Platform Method Covering pH 4-10

SCIEX
10kDa Internal Standard
Reference : ABS-A26487

For use with the IgG Purity/Heterogeneity and SDS MW Kits.

10 kDa internal standard is used as a mobility marker and all protein mobility is calculated relative to this internal standard.

SCIEX
Advanced cIEF Starter Kit
Reference : ABS-A80976

Accurate determination of a protein’s charge heterogeneity helps establish identity and stability. Capillary Isoelectric Focusing (cIEF) is a powerful technique that allows quantitative analysis of a protein’s isoelectric point (pI). In cIEF, a mixture of sample and ampholyte is introduced into a capillary and subjected to electrophoretic separation. In this process, a pH gradient through which analytes migrate to their respective pI is formed. 

The PA 800 Plus Pharmaceutical Analysis System automates the advanced cIEF technology necessary to successfully achieve high precision and quantitative separations. The use of optimized universal methods and synthetic pI markers attains the highest levels of precision in pI calculation and direct isoform quantitation with on-line UV detection.

This kit provides reagent volumes for analysis of 100 samples and includes: 
• eCap Neutral Capillary, 1 unit 
• cIEF gel 
• cIEF Peptide Marker Kit, 5 vials (pI 4.1, pI 5.5, pI 7.0, pI 9.5, pI 10.0), 240 µL each 
• Sample Loading Solution (SLS), 6 mL 
• CE Grade Water 
 
 
NOTE: Additional reagents from other vendors are required for this application. Methods and necessary supplies can be referenced in the following AIBs, available for download at www.sciex.com:
• A-11634: Identification of System Parameters Critical for High-Performance cIEF
• A-12015: A Robust cIEF Method: Intermediate Precision for the pH 5-7 Range
• A-12026: High-Resolution cIEF of Therapeutic Monoclonal Antibodies: A Platform Method Covering pH 4-10

SCIEX
Coolant for capillary cartridge liquid cooling, 450ML.
Reference : ABS-359976

Cooling liquid refill for use on temperature controlled instruments, for all Sciex CE systems including the BioPhase, PA 800 Plus, CESI 8000 Plus and P/ACE MDQ instruments (Qty: 1).

SCIEX
IgG Control Standard - 3 Pack
Reference : ABS-391734

Kit Component of P/N 390953 and P/N A10663 (IgG Purity/Heterogeneity Kit.)
For use as an experimental control when separating reduced or non-reduced IgG, with a designated quantity of non-glycosylated heavy chain to test both the resolution and quantitation suitability of the assay prior to running unknowns.

SCIEX
IgG Purity/Heterogeniety Assay Kit
Reference : ABS-A10663

Includes IgG Control Standard 1-pack and all chemistries.
The IgG Purity/Heterogeneity Assay has been developed for researchers employed in industrial biotechnology who are developing and manufacturing IgG reagents for research, diagnostic and therapeutic use. 

This assay has been specified for use on the PA 800 Plus to assess the purity and heterogeneity of IgG reagents in both a reduced and non-reduced state. The methodology involves heat-denaturing a specified concentration of IgG (both reduced and non-reduced) in the presence of SDS, and separating these proteins by size using high-resolution capillary gel electrophoresis technology. This assay will detect impurities as low as 0.1% and includes an IgG control with a designated quantity of non-glycosylated heavy chain to test both the resolution and quantitation suitability of the assay prior to running unknowns
 
Assay chemistry includes:
• Separation Capillary, 57 cm x 50 µm ID bare fused-silica, (2)
• SDS Gel Separation Buffer (proprietary formulation), 140 mL
• SDS Sample Buffer, 100 mM Tris-HCl, pH 9.0/1% SDS, 50 mL
• IgG Control Standard, 1 mg/mL in SDS sample buffer , 1 mL
• Internal Standard, 10 kDa protein, 5 mg/mL, 0.4 mL
• Acidic Wash Solution, 0.1 N HCl, 100 mL
• Basic Wash Solution, 0.1 N NaOH, 100 mL
• IgG Purity and Heterogeneity Analysis Guide, (1)

SCIEX
LIF Performance Test Mix
Reference : ABS-726022

LIF Test Mix, Sodium Fluorescein (20 mL). Test mix for testing the Laser-Induced Fluorescence (LIF) detector and determining the calibration correction factor (CCF) for the system.

SCIEX
Molecular Weight Sizing Standard - 3 Pack
Reference : ABS-A22196

Molecular Weight standards (10 to 225 KDa), 16mg/mL (100 µL) for use with the SDS-MW Kit (Part Number 390953) to provide a sizing ladder to reference proteins of interest against.

SCIEX
Pre-Assembled Capillary Cartridge
Reference : ABS-A55625

No more need to mount capillaries. Pre-assembled EZ-CE cartridge (part # A55625) for multiple applications (Purity Analysis, Rapid Charge Variant Analysis, and Fast Glycan Analysis)
> Precision cut tips – ensure uniformity of injection plug from capillary to capillary
> Alignment precision at window – reduces potential failure due to misalignment
> Reduce preparation time for operator – simplifying the process and training requirements

SCIEX
Pre-cut N-CHO Coated Capillary 50 µm x 65 cm
Reference : ABS-477601
N-CHO (PVA) Capillary with laser burned window - 50 µm ID x 65 cm total length.
Capillaries must be trimmed to optimize speed/resolution.
This capillary is used with the Carbohydrate Labeling and Analysis Kit.
Reorder component for P/N 477600.
SCIEX
Pre-cut Neutral Coated Capillary 50 µm x 67 cm total length
Reference : ABS-477441

eCAP™ Neutral Coated Capillary with laser burned window - 50 µm ID x 67 cm total length.
Capillary used with the Advanced cIEF Starter Kit (Part number A80976).
Capillaries must be trimmed to optimize for speed/resolution.
This capillary is also suitable for other applications where no EOF is desired. Thanks to the polyacrylamide coating, no EOF is present. The capillary can be used with both UV and LIF detection systems but capillary life is impacted when used with diodearray detection systems.

SCIEX
SDS-MW Analysis Kit
Reference : ABS-390953

The SDS-Gel MW Analysis Kit is designed for the separation and sizing of protein–SDS complexes using a replaceable gel matrix. The gel is formulated to provide an effective protein sieving range of approximately 10 to 225 kDa. Within this size range, the logarithm of protein molecular mass is linear with its reciprocal electrophoretic mobility, allowing the molecular weight of an unknown protein to be estimated from a standard curve of known protein sizes. This chemistry can also be used to effectively quantify the amount of protein and to determine the purity of a protein product.

This includes:
• Separation Capillary, 57 cm x 50 µm ID, bare fused-silica, (2)
• SDS Gel Separation Buffer, 140 mL
• SDS Sample Buffer, 100 mM Tris-HCl, pH 9.0/1% SDS, 50 mL
• SDS Protein Sizing Standard (10 to 225 kDa), 16 mg/mL, 100 µL
• Internal Standard, 10 kDa protein, 5 mg/mL, 0.4 mL
• Acidic Wash Solution, 0.1 N HCl, 100 mL
• Basic Wash Solution, 0.1 N NaOH, 100 mL
• SDS-Gel MW Analysis Guide (1)

SCIEX
SDS-MW Gel Buffer, Multi-Pack
Reference : ABS-A30341

SDS-MW Gel Buffer is a 4-pack of the SDS-MW gel used in the SDS-MW Kit (Part Number 390953) and the IgG Purity and Heterogeneity Kit Assay (Part Number A10663).

SDS-MW gel designed for the separation and sizing of protein samples, utilizing SDS complexes as a replaceable gel matrix.