Abbelight SMLM / TIRF Microscopie & Beeldvormingssystemen Workshop
Join us the 8th july from 14.00 for this Abbelight SMLM / TIRF workshop
This workshop will take place on Tuesday July 8th. 14:00 – 18:00.
Thanks to the support of the Erasmus MC Optical Centre, our Analis Microscopy experts are organising this workshop at the ERAMUS MC Rotterdam.
EVs are reshaping oncology, both as drivers of metastasis and as drug delivery vehicles.
But their nanoscale size makes analysis a challenge... Until now.
What’s the breakthrough?
The Abbelight SAFe platform leverages super-resolution nanoscopy to:
✅ Image thousands of individual EVs in one fast acquisition
✅Reveal molecular makeup with unmatched precision
✅Enable high-content screening with the largest field of view on the market
What you can learn during this workshop ?
Participant will learn that SMLM* is a powerful technique when applied to EV analysis due to its high spatial resolution. Detecting more than thousands of EVs in a single field of view, thanks to the Abbelight’s ASTER technique, helps in characterization and statistical analysis of EVs rapidly. Abbelight Ultimate 3D imaging is an additional powerful feature which allows to better characterize EVs shape to achieve full understanding of subpopulations.
The system that is being demonstrated is the Abbelight SAFe 360 TIRF/HiLo/SMLM microscope based on the Evident IX83 platform.
- Dual camera detection for 3D single-molecule and simultaneous multicolor imaging
- Extended axial range with nanometer accuracy
The three Abbelight nanoscopes include the same SAFe Light illumination module, which integrates ASTER technology for homogenous illumination over a 150 × 150 μm field of view and enables EPI, HiLo, or TIRF excitation modality.
Why should you join us?
To get acquainted with the technologies Abbelight offers in combination with EVIDENT IX83 inverted platform.
Who is this workshop intended for?
Imaging facility leaders, principal investigators and their PhD students.
Contact people
Vincent STEINE - ANALIS - Sales Engineer Microscopy - vincent.steine@analis.nl
Gert-Jan Cremers - Erasmus MC - Optical Imaging Centre - Head microscopy facility - g.kremers@erasmusmc.nl
*Single Molecule Localization Microscopy (SMLM) uses various techniques to cause individual fluorescent molecules to “blink.” These individual blinks are processed to produce precise, high-resolution images (down to 10nm) showing the 3D coordinates of single molecules. Using SMLM, users can access new avenues for both spatial and temporal analyses at the nanometer scale.
Multicolor Super-Resolution Imaging of COS7 Cells Using Spectral Demixing | NeuroCyto Lab
This multicolor super-resolution image captures the intricate architecture of the endoplasmic reticulum and tubulin cytoskeleton in COS7 cells, visualized using Abbelight’s SAFe 360 system.
Prepared by Karoline Friedl at the NeuroCyto Lab, this sample showcases:
- Endoplasmic reticulum labeled with AF647
- Tubulin labeled with CF680
The image was acquired using spectral demixing, a powerful technique that enables simultaneous multicolor SMLM imaging with just one laser, one buffer, and one acquisition. This method leverages ratiometric spectral intensity analysis across a large field of view (FOV), offering a clean separation of spectrally close dyes without chromatic aberration.
Technique: SAFe 360 + Spectral Demixing
Application: Subcellular structure mapping with nanometer resolution
Contributed by: Karoline Friedl, NeuroCyto Lab (France)
Want to Know more or looking for other high-end microscopy solutions?
Discover more about Abbelight SAFe Super-Resolution Nanoscopy Systems