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Spectrophotometer

What is UV-VIS spectrometry?

UV-VIS spectrophotometry is based on the principle that molecules absorb light at specific wavelengths, and this absorption is proportional to the concentration of the absorbing substance in the sample. The key equation governing this relationship is known as the Lambert-Beer Law, also referred to as Beer's Law or the Beer-Lambert Law. It describes the linear relationship between the absorption of light by a substance and its concentration in a solution.

UV-VIS Spectrometry Principle shematic diagram


The Beer-Lambert Law

The law is expressed mathematically as: A=ε⋅c⋅l
Where: 

  • A is the absorbance of the sample.
  • ε (epsilon) is the molar absorptivity or molar extinction coefficient.
  • c is the concentration of the absorbing species in the solution.
  • l is the path length of the cuvette, i.e., the distance that light travels through the sample.

Genesys Series: Flexible UV-Vis Spectrophotometry for Modern Laboratories

The Thermo Scientific™ GENESYS™ G5 series combines reliable UV-Vis performance, intuitive operation and workflow flexibility for modern laboratories.

 Engineered for accuracy and ease of use, these spectrophotometers are equipped with advanced features, ensuring optimal performance across a spectrum of applications. The robust and compact Vis and UV-Vis spectrophotometers are ideal for routine analysis, rapid measurements and demanding QA/QC environments.

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Thermo Genesys G5 UV-Vis Spectrophotometer with touch screen and chamber opened

Key benefits of the Genesys G5 series

  • Small footprint
  • Reliable optical performance for accurate and reproducible measurements
  • Affordable price and low cost of ownership
  • Available in Vis, UV-Vis and UV-Vis Pro configurations
  • UV-Vis models feature a xenon flash lamp for long lamp lifetime and reduced maintenance
  • Intuitive 7-inch high-resolution touchscreen
  • Sample compartment accommodates a wide range of flexible accessories, including multi-cell changers, Peltier temperature control, sippers and fiber optic probes
  • Modern connectivity including USB, networking and LIMS integration capabilities
  • Customizable methods and application-focused software for consistent and efficient workflows
  • Ideal for quality control, food & beverage, environmental, life science, industrial and research laboratories


The GENESYS G5 family shares accessories, software and workflows across all models, allowing laboratories to simplify training, standardize operation and adapt to changing analytical requirements.

The GENESYS G5 family is the latest evolution of the widely used GENESYS 50, 140, 150 and 180 spectrophotometers, providing a common platform for future laboratory requirements.

Thermo Genesys G5 Software supports applications in life sciences, water analysis, and food & beverage testing

Expand your UV-Vis capabilities with GENESYS G5 Software

Intuitive, flexible and easy to deploy.

GENESYS G5 Software supports applications in life sciences, water analysis, and food & beverage testing, with pre-configured methods for DNA, RNA, protein quantification and color measurement. Custom methods can be created, saved and reused to ensure consistent and reproducible analysis. 

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Evolution Series: Evolving Excellence in Spectral Analysis 

The new generation of Evolution™ UV-Vis spectrophotometers is designed to meet the requirements of pharmaceutical, industrial QA/QC, chemical and academic laboratories. 
Through the user-friendly Insight™ Pro software, it is possible to analyse your sample step by step. You can also save QA/QC procedures, making your measurements smoother. The Insight Pro Security Software helps you keep your laboratory compliant with 21 CFR Part 11, incorporating all the benefits of the Insight Pro software with added security to ensure the integrity of scientific data.
 Fixed and variable bandwidth options
 Xenon flash lamp guaranteed for up to 3 years of maintenance-free use
 Extensive range of accessory options to meet a wide array of sampling requirements
 Wide range of accessories to help measure any sample
 Comply with current 2019 USP, EP, or JP requirements
 Optional Insight Pro Security Software available to achieve 21 CFR part 11 compliance

Conclusion: Gain Precision and Reliability in Spectral Analysis with Thermo Scientific Spectrophotometers

Thermo Scientific's Genesys and Evolution series of spectrophotometers embody a legacy of excellence in spectral analysis. Whether you seek the robust performance of the Genesys series or the advanced capabilities of the Evolution series, Thermo Scientific ensures that your analytical needs are met with precision and reliability. Elevate your research and analytical processes with spectrophotometers designed to exceed expectations. 

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