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SFR vario v2 Shake Flask Reader for pH, DO, OUR and biomass

Reference: PRE-200001689

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The SFR vario offers online monitoring of oxygen, biomass and pH or CO2 - simultaneously. Online measured biomass data can be correlated with parameters like optical density, cell dry weight, or cell concentration.

This way it is possible to get real-time information on e. g. OD600 development. The device optics can read out one oxygen and one pH or CO2 (CO2 Technical Data) sensor spots - integrated in the ready-to-use cultivation vessels - and also comprise a dedicated optical set-up for biomass monitoring. The oxygen uptake rate (OUR) can be calculated from the slope of the online oxygen measurements. The system has two long-lasting, rechargeable batteries, and is compatible with any standard shaking incubators. Up to 4 SFR vario can be controlled from the SFR vario software. Measurement data are transferred wirelessly via Bluetooth to a PC / notebook.

- Simultaneous real-time measurement of O2, biomass & pH or CO2
- Automatic OUR calculation
- Online measurement of optical density, cell dry weight, cell concentration by correlation with biomass measurements
- Parallel measurements in up to 4 shake flasks
- Wireless data transfer enables easy integration
- Bioprocess development & media optimization

SFR vario v2 consists of :
- SFR vario v2
- 2 rechargeable batteries
- charge adapter cord with US / EU / UK compatible plug
- USB Bluetooth adapter for communication to PC
- screw driver and mounting screws
- Getting Started

This device comprises rechargeable batteries (accumulators) for self-contained
power supply. Rechargeable batteries may be replaced by the manufacturer only.
Rechargeable batteries are subject to normal wear which is more or less
pronounced depending on operating and storage conditions. This normal wear is
no defect which is covered by the device warranty.

    This combination does not exist.

    Technical information

    SpecificationsOxygenpH*Biomass


    Measuring range0 – 100 % O25.5 – 8.0 pHOptical Density OD600 1 - 80
    Response time (t90) at
     25 °C    		< 60 sec.< 60 sec.-
    Resolution

    ± 0.01 % O2 at 0.21 % O2
    ± 0.1 % O2 at 20.9 % O2

    		± 0.01 pH at pH = 7**Depending on culture
    Accuracy

    ± 0.05 % O2 at 0.2 % O2
    ± 0.4 % O2 at 20.9 % O2

    ± 0.1 pH at pH = 7 with one-point adjustment
    ± 0.2 pH at pH = 7 with pre-calibration

    		Depending on culture
    Drift< 0.01 % O2 per day (sampling interval of 1 min.)< 0.01 pH per day (sampling interval of 1 min.)Depending on culture

    Properties

     

     

     

    Temperature rangefrom + 5 to + 50 °C
     
    CompatibilityAqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 10
    Cross-sensitivityTypically no cross-sensitivity in culture mediaReduced to ionic strength (salinity);
    a high concentration of small fluorescent molecules
    in the visible range can interfere    		 
    Sensor flasks are delivered irradiated

    * provided Sensor Flasks are used without further handling in physiological solutions
    ** at 100 rpm & in cell culture media