Microbioreactors

Optimize Microbial Screening with Beckman Coulter Microbioreactors

The stand-alone and integrated microbioreactors from Beckman Coulter Life Sciences help to accelerate the screening of microbial strains, media, and bioprocess conditions with real-time evaluations of biomass, fluorescence, pH value, dissolved oxygen, and other key cultivation parameters.  Enjoy the benefits of automated sample draws and liquid transfers, easy data analysis, and increased walk-away time.

BioLector XT microbioreactor Beckman Coulter fermentation   
Harness Real-Time Insights with BioLector XT microbioreactor

The BioLector XT microbioreactor enables real-time evaluation of biomass, fluorescence, pH, dissolved oxygen in the liquid phase (DO), and other key cultivation parameters for aerobic, anaerobic and phototrophic microorganisms. 

Building on trusted BioLector Pro technology, the BioLector XT microbioreactor is based on a standard ANSI/SLAS (SBS) microtiter plate (MTP) format, and operates with online, pre-calibrated optical sensors. Disposable 48 well MTPs enable online measurement of cultivation parameters, while patented microfluidic technology supports simultaneous pH control and feeding. The optional microfluidic module eliminates manual liquid handling—no tubing or pipetting required, as everything is part of the gamma-radiated ready-to-use plate.

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BioLector XT Microbioreactor & Biomek i5 Liquid Handler Integration

The integration of the BioLector XT microbioreactor and a Biomek i-Series liquid handler allows you to increase your walk-away time for microbial strain and process screening experiments by unlocking the full potential of an automated screening platform: 

  • Automated sampling / harvesting
  • Automated inoculation of cultivation wells
  • Automated induction
  • Bolus feeding / repeated fed-batch
  • Induction profiling
  • Microbial growth synchronization
  • Feed profiling
  • Media preparation
  • Automated upstream processing with microbial cultures 

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BioLector XT Video Overview

This video gives a comprehensive overview of the BioLector XT instrument and components.      Watch the video gallery

Unlock the Advantages of our solutions for Your Business

Gassing head lid for microplate in BioLector

Innovative new gassing head

 Enables fed-batch experiments under anaerobic conditions
 Gassing with O2 within a range of 1%–100% and with CO2 within 1%–12%
 Reduces gas consumption to a few mL/minute
 Optional humidification of gases reduces evaporation

Microfluidic module for Biolector XT microbioreactor

Optional microfluidic module

 Unleash the full potential of the BioLector XT
 Complements the online monitoring function with well-specific pH regulation/feeding
 Enables the use of 2 reservoir wells per 4 cultivation wells
 Microvalves allot liquids at the nanolitre scale

BioLector Flowerplate Microfluidic microfermentation

“Plug-and-play” plate design

 Real-time kinetics out of 48/32 parallel cultivations
 Customizable feeding strategies (batch, fed-batch, bolus, continuous)*
 Control of pH on-the-plate with pre-calibrated optical sensors (*)
 Small working volume (800 – 2400 μL)
(*) Functionality requires optional microfluidic module

BioLector Microfermentation BioLection Software

Intelligent BioLection Software

 Intuitive user interface supports multi-user environments
 Free programming of all control parameters
 Open system enables live data downloads
 Fast processor ensures rapid download of experiment data

Advantages & Applications of the BioLector XT Microbioreactor

High-throughput microbioreactor enables real-time evaluation of biomass, fluorescence, pH, dissolved oxygen in the liquid phase (DO), and other key cultivation parameters for aerobes and anaerobes. 

gas humidification bottle replacement on Beckman Coulter Biolector XT

Building on trusted BioLector Pro technology, the BioLector XT microbioreactor is based on a standard ANSI/SLAS (SBS) microtiter plate (MTP) format and operates with online, pre-calibrated optical sensors. Disposable 48-well MTPs enable online measurement of biomass, fluorescence, pH and DO, while patented microfluidic technology supports simultaneous pH control and feeding. The optional microfluidic module eliminates manual liquid handling—no tubing or pipetting is required, as everything is part of the gamma-radiated, ready-to-use plates.

Scale-up is the bottleneck in bioprocess development. It takes a lot of time, drastically slowing down early-stage development and thus the path to the final product. From early screening experiments in shake flasks over microbial cultivation in smaller fermentors to production scale, your processes can take days or weeks. You can, of course, reduce the time by using multiple fermentors simultaneously. But this is limited by your available space, human resources, and budget. Imagine how costly it would be to run 32, or even 48, parallel fermentations in sizable fermentors …

And now imagine if you could achieve such a throughput with only two hours of hands-on time.

 Up to 48 simultaneous fermentations in a single microtiter plate.
 Comprehensive online monitoring of biomass (broad OD range), fluorescence, DO, and pH.
 NEW: Precisely controllable oxygen supply from 0-100 % O₂ thanks to gas-tight lid*
 Suitable for fed-batch cultivations (anaerobic or aerobic), thanks to well-individual pH and feeding control*.
 Automatable for complex experiment designs (DoE), sampling, and more.

  Vaccine development
   Fed-batch development*
  pH profiling*
  Feeding rate optimization*
  Media screening and optimization
  Optimization of cultivation parameters
  Cell line and strain screening
  Synthetic and systems biology
  Anaerobic / aerobic / microaerophilic cultivations
  Statistical design of experiments (DoE)
  Growth characterization
  High-throughput protein expression
  Enzyme and cell activity tests
  Functional genomics
  Proteomic studies
  Inhibition and toxicity tests
  Quality control
 

Explore In-Depth Insights: Application Notes and Publications

Ask our application notes

BioLector Microbioreactor List of Publications

BioLector Microbioreactor List of Publications.

Anaerobic cultivation processes of probiotic bacteria in the BioLector XT microbioreactor

Anaerobic cultivation processes of probiotic bacteria in the BioLector XT microbioreactor.

Using the BioLector XT Microbioreactor with the gassing lid

Using the BioLector XT Microbioreactor with the gassing lid.

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