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BioLector XT microbioreactor

Reference: BEC-M2P-G-BLXT

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High-throughput microbioreactor enables real-time evaluation of biomass, fluorescence, pH, dissolved oxygen in the liquid phase (DO), and other key cultivation parameters for aerobes and anaerobes. Building on trusted BioLector Pro technology, the BioLector XT microbioreactor is based on a standard ANSI/SLAS (SBS) microtiter plate (MTP) format, and operates with online, pre-calibrated optical sensors. Disposable 48 well MTPs enable online measurement of cultivation parameters, while patented microfluidic technology supports simultaneous pH control and feeding. The optional microfluidic module eliminates manual liquid handling—no tubing or pipetting required, as everything is part of the gamma-radiated ready-to-use plate.

The BioLector XT microbioreactor includes the following features:

  • 1x BioLector XT microbioreactor with 6 LED filter modules for the parameters biomass, pH (HP8), DO (Pst3), pH (LG1), DO (RF) and riboflavin (Ex 436 nm / Em 540 nm),
  • 1x humidification of the head space,
  • 1x 48 well MTP gassing lid (M2P-E-BD48),
  • 1x foil Applicator (M2P-F-APPLXT),
  • 1x flight case for safe transport,
  • 5x unlimited licences for the BioLection software (no PC included).

    This combination does not exist.

    Technical information

    Cultivation conditions

    TEMPERATURE

    10 – 50 °C (min. temp.: 8 °C below ambient temp.)


    SHAKING SPEED

    100 – 1500 rpm (3 mm diameter)


    ENVIRONMENTAL CONDITIONS

    Active humidification
    Ambient air
    1 – 100 % O2 (optional)
    0 – 12 % CO2 (optional)
    Anaerobic cultivation (optional)


    OXYGEN OPTODES

    0 – 100 % dissolved oxygen*1


    pH OPTODES

    pH 4 – 7.5 (depending on plate)


    MTP READING TIME

    2.7 min / filter / 48 wells @ 1000 rpm

    Microfluidic features*2

    TRIGGERED pH CONTROL (CLOSED LOOP CONTROLLER)

    pH control range: 4.0 – 7.5 (depending on plate)
    Fully editable PI control
    Slow, medium and fast PI default settings


    FEEDING OPTIONS

    Two sided pH control (alkali and acid)
    One sided pH control and one feed line (alkali or acid + one feed)
    Two feed lines


    FEEDING PROFILES

    Profile equation: dV/dt =A+B×t+C×eD×t
    Constant: A [μL/h]
    Linear: A [μL/h] and B [μL/h²]
    Exponential: A [μL/h], B [μL/h²], C [μL/h] and D [h-1]
    Pulse feed
    Full feeding profile flexibility permits broad experimental design


    PUMP RATE

    Up to 665 pump strokes per hour