What is steam sterilization?
Steam sterilization is a method employed in the field of sterilization using a steam sterilizer or autoclave. The process is designed to eliminate all types of microbial life, including potentially harmful agents like fungi, bacteria, viruses, and spores. The method can be applied to sterilize surfaces, fluids, medications, or compounds, such as biological culture media.
The procedure of steam sterilization is executed under specific conditions, including a steam pressure ranging from 1 to 5 bar, and a sterilization temperature within the range of 100 to 150°C.
Commonly applied parameters for steam sterilization include:
- 121°C for 15 to 20 minutes
- 134°C for 3 to 5 minutes
These standardized parameters ensure the effective elimination of microbial life, meeting the stringent requirements of sterilization in various applications.

SYSTEC V SERIES
Vertical floor-standing autoclaves (top-loading) from 40 to 150 liters chamber volume

SYSTEC D SERIES
Horizontal bench-top autoclaves (front loading) from 23 to 200 liters chamber volume


SYSTEC H & H-2 SERIES
H Series: horizontal high capacity autoclaves (front-loading) from 65 zo 1580 liters chamber volume
H-2 Series: double-door (pass through) autoclaves from 90 to 1580 liters chamber volume
What are the advantages of having your steam generator outside the pressure chamber?
Steam can either be generated by internal heating elements inside or outside the pressure vessel of the autoclave. When the steam generator is outside the pressure vessel, it is still integrated in the housing of the autoclave. Having the steam generator outside the pressure vessel comes with some advantages:
- Much better process control
- Faster heating up to time
- Vacuum can be pulled to ensure 100% removal of air, especially when sterilizing hollow items and waste
- Volume of the external steam generator is smaller and and often does not require a 3rd party for a yearly validation
- Easier to clean the pressure vessel
What is a media preparator?
A mediaprep or media preparator is a device very similar to an autoclave, but designed specifically for media preparation. The medium is prepared (stirrer integrated in the vessel) directly into the pressure vessel and can be pumped directly to a filling station. The advantages of using a mediaprep for media preparations:
- Mediapreps are optimized for fast and save media sterilization
- Preparation and sterilization are done in one process
- The filling of the culture media can be automized
- Reduced risk of re-contamination of the culture media after sterilisation
Systec Mediaprep & Systec Mediafill Solutions

Have a look at this video showing a culture media sterilizer & automated petri-dish plate-pourer. Systec Mediaprep agar preparator and sterilizer and Systec Mediafill automated plate-pourer for petri-dishes, bi-plates, tri-plates and test-tubes. Fully automatic dosing and filling of agar into petri-dishes (Ø 90, 70, 60 or 35 mm) and test tubes.
Analis is an exclusive distributor for Systec autoclaves and media preparators for Belgium ! Ask our experts for more information or support. Contact us
You may also need Air Samplers and Biosafety Cabinets
Our products
- Advanced HMI and PLC
with touch-screen control
- Optimized HMI allows
quick navigation and customization of processes and settings
- Newly developed autoclave system software (PLC)
- Code-secured access rights for changing parameters and further
safety-relevant intervention
- Internal memory capacity for storing minimum 10 years of documentation
data
- Timer for programmable start time
- Calculation of F0 value
- Adjustable automatic opening of door at end of program
- Network (Ethernet) and USB ports for external data transfer
- Text input for batch information (up to 20 characters)
- Integrated feature for comprehensive batch documentation, with export as
PDF and CSV either on a USB-Stick or into the Ethernet network
- Backup feature allows to backup and restore the complete autoclave software
including sterilization programs, individual settings and parameters
- Time synchronization
with time server via network or internet
- Integrated steam generator separated from the chamber
- Housing, support frame and pressure chamber, made of corrosion-resistant
stainless steel
- The pressure vessel of the autoclave is made of corrosion-resistant
stainless steel 1.4404 (AISI 316L) and is electro-polished for ease of cleaning
- Ring-lock system for safe locking of the pressure vessel door
- All connections on the pressure vessel are designed as Tri-Clamp
- Temperature and pressure range up to 140 °C, 4 bar absolute pressure,
optionally extendable to 150°C, 5 bar
- Up to 100 sterilization programs, sterilization programs and program
list fully customizable
- 2x flexible PT-100 temperature sensors (Class A) in the autoclave
chamber
- Additional temperature sensor in condensate exhaust
- Water-cooled waste water exhaust, thermostatically controlled
- Validation port for introducing external measuring equipment
- Advanced HMI and PLC with touch-screen control
- Optimized HMI allows quick navigation and customization of processes and settings
- Newly developed autoclave system software (PLC)
- Code-secured access rights for changing parameters and further safety-relevant intervention
- Internal memory capacity for storing minimum 10 years of documentation data
- Timer for programmable start time
- Calculation of F0 value
- Adjustable automatic opening of door at end of program
- Network (Ethernet) and USB ports for external data transfer
- Text input for batch information (up to 20 characters)
- Integrated feature for comprehensive batch documentation, with export as PDF and CSV either on a USB-Stick or into the Ethernet network
- Backup feature allows to backup and restore the complete autoclave software including sterilization programs, individual settings and parameters
- Time synchronization with time server via network or internet
- Integrated steam generator separated from the chamber
- Housing, support frame and pressure chamber, made of corrosion-resistant stainless steel
- The pressure vessel of the autoclave is made of corrosion-resistant stainless steel 1.4404 (AISI 316L) and is electro-polished for ease of cleaning
- Ring-lock system for safe locking of the pressure vessel door
- All connections on the pressure vessel are designed as Tri-Clamp
- Temperature and pressure range up to 140 °C, 4 bar absolute pressure, optionally extendable to 150°C, 5 bar
- Up to 100 sterilization programs, sterilization programs and program list fully customizable
- 2x flexible PT-100 temperature sensors (Class A) in the autoclave chamber
- Additional temperature sensor in condensate exhaust
- Water-cooled waste water exhaust, thermostatically controlled
- Validation port for introducing external measuring equipment
- Advanced HMI and PLC with touch-screen control
- Optimized HMI allows quick navigation and customization of processes and settings
- Newly developed autoclave system software (PLC)
- Code-secured access rights for changing parameters and further safety-relevant intervention
- Internal memory capacity for storing minimum 10 years of documentation data
- Timer for programmable start time
- Calculation of F0 value
- Adjustable automatic opening of door at end of program
- Network (Ethernet) and USB ports for external data transfer
- Text input for batch information (up to 20 characters)
- Integrated feature for comprehensive batch documentation, with export as PDF and CSV either on a USB-Stick or into the Ethernet network
- Backup feature allows to backup and restore the complete autoclave software including sterilization programs, individual settings and parameters
- Time synchronization with time server via network or internet
- Integrated steam generator separated from the chamber
- Housing, support frame and pressure chamber, made of corrosion-resistant stainless steel
- The pressure vessel of the autoclave is made of corrosion-resistant stainless steel 1.4404 (AISI 316L) and is electro-polished for ease of cleaning
- Ring-lock system for safe locking of the pressure vessel door
- All connections on the pressure vessel are designed as Tri-Clamp
- Temperature and pressure range up to 140 °C, 4 bar absolute pressure, optionally extendable to 150°C, 5 bar
- Up to 100 sterilization programs, sterilization programs and program list fully customizable
- 2x flexible PT-100 temperature sensors (Class A) in the autoclave chamber
- Additional temperature sensor in condensate exhaust
- Water-cooled waste water exhaust, thermostatically controlled
- Validation port for introducing external measuring equipment
• Advanced HMI and PLC with touch-screen control
• Optimized HMI allows quick navigation and customization of processes and settings
• Newly developed autoclave system software (PLC)
• Code-secured access rights for changing parameters and further safety-relevant intervention
• Internal memory capacity for storing minimum 10 years of documentation data
• Timer for programmable start time
• Calculation of F0 value
• Adjustable automatic opening of door at end of program
• Network (Ethernet) and USB ports for external data transfer
• Text input for batch information (up to 20 characters)
• Integrated feature for comprehensive batch documentation, with export as PDF and CSV either on a USB-Stick or into the Ethernet network
• Backup feature allows to backup and restore the complete autoclave software including sterilization programs, individual settings and parameters
• Time synchronization with time server via network or internet
• Integrated steam generator separated from the chamber
• Housing, support frame and pressure chamber, made of corrosion-resistant stainless steel
• The pressure vessel of the autoclave is made of corrosion-resistant stainless steel 1.4404 (AISI 316L) and is electro-polished for ease of cleaning
• Ring-lock system for safe locking of the pressure vessel door
• All connections on the pressure vessel are designed as Tri-Clamp
• Temperature and pressure range up to 140 °C, 4 bar absolute pressure, optionally extendable to 150°C, 5 bar
• Up to 100 sterilization programs, sterilization programs and program list fully customizable
• 2x flexible PT-100 temperature sensors (Class A) in the autoclave chamber
• Additional temperature sensor in condensate exhaust
• Water-cooled waste water exhaust, thermostatically controlled
• Validation port for introducing external measuring equipment
• Advanced HMI and PLC with touch-screen control
• Optimized HMI allows quick navigation and customization of processes and settings
• Newly developed autoclave system software (PLC)
• Code-secured access rights for changing parameters and further safety-relevant intervention
• Internal memory capacity for storing minimum 10 years of documentation data
• Timer for programmable start time
• Calculation of F0 value
• Adjustable automatic opening of door at end of program
• Network (Ethernet) and USB ports for external data transfer
• Text input for batch information (up to 20 characters)
• Integrated feature for comprehensive batch documentation, with export as PDF and CSV either on a USB-Stick or into the Ethernet network
• Backup feature allows to backup and restore the complete autoclave software including sterilization programs, individual settings and parameters
• Time synchronization with time server via network or internet
• Integrated steam generator separated from the chamber
• Housing, support frame and pressure chamber, made of corrosion-resistant stainless steel
• The pressure vessel of the autoclave is made of corrosion-resistant stainless steel 1.4404 (AISI 316L) and is electro-polished for ease of cleaning
• Ring-lock system for safe locking of the pressure vessel door
• All connections on the pressure vessel are designed as Tri-Clamp
• Temperature and pressure range up to 140 °C, 4 bar absolute pressure, optionally extendable to 150°C, 5 bar
• Up to 100 sterilization programs, sterilization programs and program list fully customizable
• 2x flexible PT-100 temperature sensors (Class A) in the autoclave chamber
• Additional temperature sensor in condensate exhaust
• Water-cooled waste water exhaust, thermostatically controlled
• Validation port for introducing external measuring equipment
• Advanced HMI and PLC with touch-screen control
• Optimized HMI allows quick navigation and customization of processes and settings
• Newly developed autoclave system software (PLC)
• Code-secured access rights for changing parameters and further safety-relevant intervention
• Internal memory capacity for storing minimum 10 years of documentation data
• Timer for programmable start time
• Calculation of F0 value
• Adjustable automatic opening of door at end of program
• Network (Ethernet) and USB ports for external data transfer
• Text input for batch information (up to 20 characters)
• Integrated feature for comprehensive batch documentation, with export as PDF and CSV either on a USB-Stick or into the Ethernet network
• Backup feature allows to backup and restore the complete autoclave software including sterilization programs, individual settings and parameters
• Time synchronization with time server via network or internet
• Integrated steam generator separated from the chamber
• Housing, support frame and pressure chamber, made of corrosion-resistant stainless steel
• The pressure vessel of the autoclave is made of corrosion-resistant stainless steel 1.4404 (AISI 316L) and is electro-polished for ease of cleaning
• Ring-lock system for safe locking of the pressure vessel door
• All connections on the pressure vessel are designed as Tri-Clamp
• Temperature and pressure range up to 140 °C, 4 bar absolute pressure, optionally extendable to 150°C, 5 bar
• Up to 100 sterilization programs, sterilization programs and program list fully customizable
• 2x flexible PT-100 temperature sensors (Class A) in the autoclave chamber
• Additional temperature sensor in condensate exhaust
• Water-cooled waste water exhaust, thermostatically controlled
• Validation port for introducing external measuring equipment
- Advanced HMI and PLC with touch-screen control
- Optimized HMI allows quick navigation and customization of processes and settings
- Newly developed autoclave system software (PLC)
- Code-secured access rights for changing parameters and further safety-relevant intervention
- Internal memory capacity for storing minimum 10 years of documentation data
- Timer for programmable start time
- Calculation of F0 value
- Adjustable automatic opening of door at end of program
- Network (Ethernet) and USB ports for external data transfer
- Text input for batch information (up to 20 characters)
- Integrated feature for comprehensive batch documentation, with export as PDF and CSV either on a USB-Stick or into the Ethernet network
- Backup feature allows to backup and restore the complete autoclave software including sterilization programs, individual settings and parameters
- Time synchronization with time server via network or internet
- Integrated steam generator separated from the chamber
- Housing, support frame and pressure chamber, made of corrosion-resistant stainless steel
- The pressure vessel of the autoclave is made of corrosion-resistant stainless steel 1.4404 (AISI 316L) and is electro-polished for ease of cleaning
- Ring-lock system for safe locking of the pressure vessel door
- All connections on the pressure vessel are designed as Tri-Clamp
- Temperature and pressure range up to 140 °C, 4 bar absolute pressure, optionally extendable to 150°C, 5 bar
- Up to 100 sterilization programs, sterilization programs and program list fully customizable
- 2x flexible PT-100 temperature sensors (Class A) in the autoclave chamber
- Additional temperature sensor in condensate exhaust
- Water-cooled waste water exhaust, thermostatically controlled
- Validation port for introducing external measuring equipment
• Advanced HMI and PLC with touch-screen control
• Optimized HMI allows quick navigation and customization of processes and settings
• Newly developed autoclave system software (PLC)
• Code-secured access rights for changing parameters and further safety-relevant intervention
• Internal memory capacity for storing minimum 10 years of documentation data
• Timer for programmable start time
• Calculation of F0 value
• Adjustable automatic opening of door at end of program
• Network (Ethernet) and USB ports for external data transfer
• Text input for batch information (up to 20 characters)
• Integrated feature for comprehensive batch documentation, with export as PDF and CSV either on a USB-Stick or into the Ethernet network
• Backup feature allows to backup and restore the complete autoclave software including sterilization programs, individual settings and parameters
• Time synchronization with time server via network or internet
• Integrated steam generator separated from the chamber
• Housing, support frame and pressure chamber, made of corrosion-resistant stainless steel
• The pressure vessel of the autoclave is made of corrosion-resistant stainless steel 1.4404 (AISI 316L) and is electro-polished for ease of cleaning
• Ring-lock system for safe locking of the pressure vessel door
• All connections on the pressure vessel are designed as Tri-Clamp
• Temperature and pressure range up to 140 °C, 4 bar absolute pressure, optionally extendable to 150°C, 5 bar
• Up to 100 sterilization programs, sterilization programs and program list fully customizable
• 2x flexible PT-100 temperature sensors (Class A) in the autoclave chamber
• Additional temperature sensor in condensate exhaust
• Water-cooled waste water exhaust, thermostatically controlled
• Validation port for introducing external measuring equipment
• Advanced HMI and PLC with touch-screen control
• Optimized HMI allows quick navigation and customization of processes and settings
• Newly developed autoclave system software (PLC)
• Code-secured access rights for changing parameters and further safety-relevant intervention
• Internal memory capacity for storing minimum 10 years of documentation data
• Timer for programmable start time
• Calculation of F0 value
• Adjustable automatic opening of door at end of program
• Network (Ethernet) and USB ports for external data transfer
• Text input for batch information (up to 20 characters)
• Integrated feature for comprehensive batch documentation, with export as PDF and CSV either on a USB-Stick or into the Ethernet network
• Backup feature allows to backup and restore the complete autoclave software including sterilization programs, individual settings and parameters
• Time synchronization with time server via network or internet
• Integrated steam generator separated from the chamber
• Housing, support frame and pressure chamber, made of corrosion-resistant stainless steel
• The pressure vessel of the autoclave is made of corrosion-resistant stainless steel 1.4404 (AISI 316L) and is electro-polished for ease of cleaning
• Ring-lock system for safe locking of the pressure vessel door
• All connections on the pressure vessel are designed as Tri-Clamp
• Temperature and pressure range up to 140 °C, 4 bar absolute pressure, optionally extendable to 150°C, 5 bar
• Up to 100 sterilization programs, sterilization programs and program list fully customizable
• 2x flexible PT-100 temperature sensors (Class A) in the autoclave chamber
• Additional temperature sensor in condensate exhaust
• Water-cooled waste water exhaust, thermostatically controlled
• Validation port for introducing external measuring equipment
• Advanced HMI and PLC with touch-screen control
• Optimized HMI allows quick navigation and customization of processes and settings
• Newly developed autoclave system software (PLC)
• Code-secured access rights for changing parameters and further safety-relevant intervention
• Internal memory capacity for storing minimum 10 years of documentation data
• Timer for programmable start time
• Calculation of F0 value
• Adjustable automatic opening of door at end of program
• Network (Ethernet) and USB ports for external data transfer
• Text input for batch information (up to 20 characters)
• Integrated feature for comprehensive batch documentation, with export as PDF and CSV either on a USB-Stick or into the Ethernet network
• Backup feature allows to backup and restore the complete autoclave software including sterilization programs, individual settings and parameters
• Time synchronization with time server via network or internet
• Integrated steam generator separated from the chamber
• Housing, support frame and pressure chamber, made of corrosion-resistant stainless steel
• The pressure vessel of the autoclave is made of corrosion-resistant stainless steel 1.4404 (AISI 316L) and is electro-polished for ease of cleaning
• Ring-lock system for safe locking of the pressure vessel door
• All connections on the pressure vessel are designed as Tri-Clamp
• Temperature and pressure range up to 140 °C, 4 bar absolute pressure, optionally extendable to 150°C, 5 bar
• Up to 100 sterilization programs, sterilization programs and program list fully customizable
• 2x flexible PT-100 temperature sensors (Class A) in the autoclave chamber
• Additional temperature sensor in condensate exhaust
• Water-cooled waste water exhaust, thermostatically controlled
• Validation port for introducing external measuring equipment