What is steam sterilization?
Steam sterilization uses a steam sterilizer, or autoclave, to eliminate all forms of microbial life — including fungi, bacteria, viruses and spores. It can sterilize surfaces, fluids, medications and compounds such as biological culture media.
The process runs under set conditions: steam pressure of 1 to 5 bar and a sterilization temperature between 100 and 150 °C.
Common sterilization parameters:
- 121 °C for 15 to 20 minutes
- 134 °C for 3 to 5 minutes
These standardized parameters ensure effective elimination of microbial life and meet strict sterilization requirements across applications.
SYSTEC V SERIES
Vertical floor-standing autoclaves (top-loading), chamber volume from 40 to 150 liters
SYSTEC D SERIES
Horizontal bench-top autoclaves (front-loading), chamber volume from 23 to 200 liters
SYSTEC H & H-2 SERIES
H Series: horizontal high capacity autoclaves (front-loading) from 65 zo 1580 liters chamber volume
H-2 Series: double-door (pass through) autoclaves from 90 to 1580 liters chamber volume
What are the advantages of a steam generator outside the pressure chamber?
Steam can be generated by heating elements either inside or outside the autoclave's pressure vessel. When the steam generator is outside the pressure vessel, it remains integrated in the autoclave housing. Placing it outside offers several advantages:
- Much better process control
- Faster heat-up time
- Vacuum can be pulled to ensure 100% air removal, especially when sterilizing hollow items and waste
- The external steam generator has a smaller volume and often does not require a third party for yearly validation
- Easier to clean the pressure vessel
What is a media preparator?
A mediaprep, or media preparator, is a device very similar to an autoclave but designed specifically for media preparation. The medium is prepared directly in the pressure vessel (with an integrated stirrer) and can be pumped straight to a filling station. Advantages of using a mediaprep:
- Mediapreps are optimized for fast, safe media sterilization
- Preparation and sterilization are done in a single process
- Culture media filling can be automated
- Reduced risk of re-contamination of the culture media after sterilization
Systec Mediaprep & Systec Mediafill Solutions
Watch this video of a culture media sterilizer and automated petri-dish plate-pourer: the Systec Mediaprep agar preparator and sterilizer and the Systec Mediafill automated plate-pourer for petri dishes, bi-plates, tri-plates and test tubes. It automatically doses and fills agar into petri dishes (Ø 90, 70, 60 or 35 mm) and test tubes.
Analis is the exclusive distributor of Systec autoclaves and media preparators for Belgium. Ask our experts for more information or support.
You may also need Air Samplers and Biosafety Cabinets
Our products
- Advanced HMI and PLC
with touch-screen control
- Optimized HMI allows
quick navigation and customization of processes and settings
- Newly developed autoclave system software (PLC)
- Code-secured access rights for changing parameters and further
safety-relevant intervention
- Internal memory capacity for storing minimum 10 years of documentation
data
- Timer for programmable start time
- Calculation of F0 value
- Adjustable automatic opening of door at end of program
- Network (Ethernet) and USB ports for external data transfer
- Text input for batch information (up to 20 characters)
- Integrated feature for comprehensive batch documentation, with export as
PDF and CSV either on a USB-Stick or into the Ethernet network
- Backup feature allows to backup and restore the complete autoclave software
including sterilization programs, individual settings and parameters
- Time synchronization
with time server via network or internet
- Integrated steam generator separated from the chamber
- Housing, support frame and pressure chamber, made of corrosion-resistant
stainless steel
- The pressure vessel of the autoclave is made of corrosion-resistant
stainless steel 1.4404 (AISI 316L) and is electro-polished for ease of cleaning
- Ring-lock system for safe locking of the pressure vessel door
- All connections on the pressure vessel are designed as Tri-Clamp
- Temperature and pressure range up to 140 °C, 4 bar absolute pressure,
optionally extendable to 150°C, 5 bar
- Up to 100 sterilization programs, sterilization programs and program
list fully customizable
- 2x flexible PT-100 temperature sensors (Class A) in the autoclave
chamber
- Additional temperature sensor in condensate exhaust
- Water-cooled waste water exhaust, thermostatically controlled
- Validation port for introducing external measuring equipment
- Advanced HMI and PLC with touch-screen control
- Optimized HMI allows quick navigation and customization of processes and settings
- Newly developed autoclave system software (PLC)
- Code-secured access rights for changing parameters and further safety-relevant intervention
- Internal memory capacity for storing minimum 10 years of documentation data
- Timer for programmable start time
- Calculation of F0 value
- Adjustable automatic opening of door at end of program
- Network (Ethernet) and USB ports for external data transfer
- Text input for batch information (up to 20 characters)
- Integrated feature for comprehensive batch documentation, with export as PDF and CSV either on a USB-Stick or into the Ethernet network
- Backup feature allows to backup and restore the complete autoclave software including sterilization programs, individual settings and parameters
- Time synchronization with time server via network or internet
- Integrated steam generator separated from the chamber
- Housing, support frame and pressure chamber, made of corrosion-resistant stainless steel
- The pressure vessel of the autoclave is made of corrosion-resistant stainless steel 1.4404 (AISI 316L) and is electro-polished for ease of cleaning
- Ring-lock system for safe locking of the pressure vessel door
- All connections on the pressure vessel are designed as Tri-Clamp
- Temperature and pressure range up to 140 °C, 4 bar absolute pressure, optionally extendable to 150°C, 5 bar
- Up to 100 sterilization programs, sterilization programs and program list fully customizable
- 2x flexible PT-100 temperature sensors (Class A) in the autoclave chamber
- Additional temperature sensor in condensate exhaust
- Water-cooled waste water exhaust, thermostatically controlled
- Validation port for introducing external measuring equipment
- Advanced HMI and PLC with touch-screen control
- Optimized HMI allows quick navigation and customization of processes and settings
- Newly developed autoclave system software (PLC)
- Code-secured access rights for changing parameters and further safety-relevant intervention
- Internal memory capacity for storing minimum 10 years of documentation data
- Timer for programmable start time
- Calculation of F0 value
- Adjustable automatic opening of door at end of program
- Network (Ethernet) and USB ports for external data transfer
- Text input for batch information (up to 20 characters)
- Integrated feature for comprehensive batch documentation, with export as PDF and CSV either on a USB-Stick or into the Ethernet network
- Backup feature allows to backup and restore the complete autoclave software including sterilization programs, individual settings and parameters
- Time synchronization with time server via network or internet
- Integrated steam generator separated from the chamber
- Housing, support frame and pressure chamber, made of corrosion-resistant stainless steel
- The pressure vessel of the autoclave is made of corrosion-resistant stainless steel 1.4404 (AISI 316L) and is electro-polished for ease of cleaning
- Ring-lock system for safe locking of the pressure vessel door
- All connections on the pressure vessel are designed as Tri-Clamp
- Temperature and pressure range up to 140 °C, 4 bar absolute pressure, optionally extendable to 150°C, 5 bar
- Up to 100 sterilization programs, sterilization programs and program list fully customizable
- 2x flexible PT-100 temperature sensors (Class A) in the autoclave chamber
- Additional temperature sensor in condensate exhaust
- Water-cooled waste water exhaust, thermostatically controlled
- Validation port for introducing external measuring equipment
• Advanced HMI and PLC with touch-screen control
• Optimized HMI allows quick navigation and customization of processes and settings
• Newly developed autoclave system software (PLC)
• Code-secured access rights for changing parameters and further safety-relevant intervention
• Internal memory capacity for storing minimum 10 years of documentation data
• Timer for programmable start time
• Calculation of F0 value
• Adjustable automatic opening of door at end of program
• Network (Ethernet) and USB ports for external data transfer
• Text input for batch information (up to 20 characters)
• Integrated feature for comprehensive batch documentation, with export as PDF and CSV either on a USB-Stick or into the Ethernet network
• Backup feature allows to backup and restore the complete autoclave software including sterilization programs, individual settings and parameters
• Time synchronization with time server via network or internet
• Integrated steam generator separated from the chamber
• Housing, support frame and pressure chamber, made of corrosion-resistant stainless steel
• The pressure vessel of the autoclave is made of corrosion-resistant stainless steel 1.4404 (AISI 316L) and is electro-polished for ease of cleaning
• Ring-lock system for safe locking of the pressure vessel door
• All connections on the pressure vessel are designed as Tri-Clamp
• Temperature and pressure range up to 140 °C, 4 bar absolute pressure, optionally extendable to 150°C, 5 bar
• Up to 100 sterilization programs, sterilization programs and program list fully customizable
• 2x flexible PT-100 temperature sensors (Class A) in the autoclave chamber
• Additional temperature sensor in condensate exhaust
• Water-cooled waste water exhaust, thermostatically controlled
• Validation port for introducing external measuring equipment
• Advanced HMI and PLC with touch-screen control
• Optimized HMI allows quick navigation and customization of processes and settings
• Newly developed autoclave system software (PLC)
• Code-secured access rights for changing parameters and further safety-relevant intervention
• Internal memory capacity for storing minimum 10 years of documentation data
• Timer for programmable start time
• Calculation of F0 value
• Adjustable automatic opening of door at end of program
• Network (Ethernet) and USB ports for external data transfer
• Text input for batch information (up to 20 characters)
• Integrated feature for comprehensive batch documentation, with export as PDF and CSV either on a USB-Stick or into the Ethernet network
• Backup feature allows to backup and restore the complete autoclave software including sterilization programs, individual settings and parameters
• Time synchronization with time server via network or internet
• Integrated steam generator separated from the chamber
• Housing, support frame and pressure chamber, made of corrosion-resistant stainless steel
• The pressure vessel of the autoclave is made of corrosion-resistant stainless steel 1.4404 (AISI 316L) and is electro-polished for ease of cleaning
• Ring-lock system for safe locking of the pressure vessel door
• All connections on the pressure vessel are designed as Tri-Clamp
• Temperature and pressure range up to 140 °C, 4 bar absolute pressure, optionally extendable to 150°C, 5 bar
• Up to 100 sterilization programs, sterilization programs and program list fully customizable
• 2x flexible PT-100 temperature sensors (Class A) in the autoclave chamber
• Additional temperature sensor in condensate exhaust
• Water-cooled waste water exhaust, thermostatically controlled
• Validation port for introducing external measuring equipment
• Advanced HMI and PLC with touch-screen control
• Optimized HMI allows quick navigation and customization of processes and settings
• Newly developed autoclave system software (PLC)
• Code-secured access rights for changing parameters and further safety-relevant intervention
• Internal memory capacity for storing minimum 10 years of documentation data
• Timer for programmable start time
• Calculation of F0 value
• Adjustable automatic opening of door at end of program
• Network (Ethernet) and USB ports for external data transfer
• Text input for batch information (up to 20 characters)
• Integrated feature for comprehensive batch documentation, with export as PDF and CSV either on a USB-Stick or into the Ethernet network
• Backup feature allows to backup and restore the complete autoclave software including sterilization programs, individual settings and parameters
• Time synchronization with time server via network or internet
• Integrated steam generator separated from the chamber
• Housing, support frame and pressure chamber, made of corrosion-resistant stainless steel
• The pressure vessel of the autoclave is made of corrosion-resistant stainless steel 1.4404 (AISI 316L) and is electro-polished for ease of cleaning
• Ring-lock system for safe locking of the pressure vessel door
• All connections on the pressure vessel are designed as Tri-Clamp
• Temperature and pressure range up to 140 °C, 4 bar absolute pressure, optionally extendable to 150°C, 5 bar
• Up to 100 sterilization programs, sterilization programs and program list fully customizable
• 2x flexible PT-100 temperature sensors (Class A) in the autoclave chamber
• Additional temperature sensor in condensate exhaust
• Water-cooled waste water exhaust, thermostatically controlled
• Validation port for introducing external measuring equipment
- Advanced HMI and PLC with touch-screen control
- Optimized HMI allows quick navigation and customization of processes and settings
- Newly developed autoclave system software (PLC)
- Code-secured access rights for changing parameters and further safety-relevant intervention
- Internal memory capacity for storing minimum 10 years of documentation data
- Timer for programmable start time
- Calculation of F0 value
- Adjustable automatic opening of door at end of program
- Network (Ethernet) and USB ports for external data transfer
- Text input for batch information (up to 20 characters)
- Integrated feature for comprehensive batch documentation, with export as PDF and CSV either on a USB-Stick or into the Ethernet network
- Backup feature allows to backup and restore the complete autoclave software including sterilization programs, individual settings and parameters
- Time synchronization with time server via network or internet
- Integrated steam generator separated from the chamber
- Housing, support frame and pressure chamber, made of corrosion-resistant stainless steel
- The pressure vessel of the autoclave is made of corrosion-resistant stainless steel 1.4404 (AISI 316L) and is electro-polished for ease of cleaning
- Ring-lock system for safe locking of the pressure vessel door
- All connections on the pressure vessel are designed as Tri-Clamp
- Temperature and pressure range up to 140 °C, 4 bar absolute pressure, optionally extendable to 150°C, 5 bar
- Up to 100 sterilization programs, sterilization programs and program list fully customizable
- 2x flexible PT-100 temperature sensors (Class A) in the autoclave chamber
- Additional temperature sensor in condensate exhaust
- Water-cooled waste water exhaust, thermostatically controlled
- Validation port for introducing external measuring equipment
• Advanced HMI and PLC with touch-screen control
• Optimized HMI allows quick navigation and customization of processes and settings
• Newly developed autoclave system software (PLC)
• Code-secured access rights for changing parameters and further safety-relevant intervention
• Internal memory capacity for storing minimum 10 years of documentation data
• Timer for programmable start time
• Calculation of F0 value
• Adjustable automatic opening of door at end of program
• Network (Ethernet) and USB ports for external data transfer
• Text input for batch information (up to 20 characters)
• Integrated feature for comprehensive batch documentation, with export as PDF and CSV either on a USB-Stick or into the Ethernet network
• Backup feature allows to backup and restore the complete autoclave software including sterilization programs, individual settings and parameters
• Time synchronization with time server via network or internet
• Integrated steam generator separated from the chamber
• Housing, support frame and pressure chamber, made of corrosion-resistant stainless steel
• The pressure vessel of the autoclave is made of corrosion-resistant stainless steel 1.4404 (AISI 316L) and is electro-polished for ease of cleaning
• Ring-lock system for safe locking of the pressure vessel door
• All connections on the pressure vessel are designed as Tri-Clamp
• Temperature and pressure range up to 140 °C, 4 bar absolute pressure, optionally extendable to 150°C, 5 bar
• Up to 100 sterilization programs, sterilization programs and program list fully customizable
• 2x flexible PT-100 temperature sensors (Class A) in the autoclave chamber
• Additional temperature sensor in condensate exhaust
• Water-cooled waste water exhaust, thermostatically controlled
• Validation port for introducing external measuring equipment
• Advanced HMI and PLC with touch-screen control
• Optimized HMI allows quick navigation and customization of processes and settings
• Newly developed autoclave system software (PLC)
• Code-secured access rights for changing parameters and further safety-relevant intervention
• Internal memory capacity for storing minimum 10 years of documentation data
• Timer for programmable start time
• Calculation of F0 value
• Adjustable automatic opening of door at end of program
• Network (Ethernet) and USB ports for external data transfer
• Text input for batch information (up to 20 characters)
• Integrated feature for comprehensive batch documentation, with export as PDF and CSV either on a USB-Stick or into the Ethernet network
• Backup feature allows to backup and restore the complete autoclave software including sterilization programs, individual settings and parameters
• Time synchronization with time server via network or internet
• Integrated steam generator separated from the chamber
• Housing, support frame and pressure chamber, made of corrosion-resistant stainless steel
• The pressure vessel of the autoclave is made of corrosion-resistant stainless steel 1.4404 (AISI 316L) and is electro-polished for ease of cleaning
• Ring-lock system for safe locking of the pressure vessel door
• All connections on the pressure vessel are designed as Tri-Clamp
• Temperature and pressure range up to 140 °C, 4 bar absolute pressure, optionally extendable to 150°C, 5 bar
• Up to 100 sterilization programs, sterilization programs and program list fully customizable
• 2x flexible PT-100 temperature sensors (Class A) in the autoclave chamber
• Additional temperature sensor in condensate exhaust
• Water-cooled waste water exhaust, thermostatically controlled
• Validation port for introducing external measuring equipment
• Advanced HMI and PLC with touch-screen control
• Optimized HMI allows quick navigation and customization of processes and settings
• Newly developed autoclave system software (PLC)
• Code-secured access rights for changing parameters and further safety-relevant intervention
• Internal memory capacity for storing minimum 10 years of documentation data
• Timer for programmable start time
• Calculation of F0 value
• Adjustable automatic opening of door at end of program
• Network (Ethernet) and USB ports for external data transfer
• Text input for batch information (up to 20 characters)
• Integrated feature for comprehensive batch documentation, with export as PDF and CSV either on a USB-Stick or into the Ethernet network
• Backup feature allows to backup and restore the complete autoclave software including sterilization programs, individual settings and parameters
• Time synchronization with time server via network or internet
• Integrated steam generator separated from the chamber
• Housing, support frame and pressure chamber, made of corrosion-resistant stainless steel
• The pressure vessel of the autoclave is made of corrosion-resistant stainless steel 1.4404 (AISI 316L) and is electro-polished for ease of cleaning
• Ring-lock system for safe locking of the pressure vessel door
• All connections on the pressure vessel are designed as Tri-Clamp
• Temperature and pressure range up to 140 °C, 4 bar absolute pressure, optionally extendable to 150°C, 5 bar
• Up to 100 sterilization programs, sterilization programs and program list fully customizable
• 2x flexible PT-100 temperature sensors (Class A) in the autoclave chamber
• Additional temperature sensor in condensate exhaust
• Water-cooled waste water exhaust, thermostatically controlled
• Validation port for introducing external measuring equipment
